Koeberle SC, Kipp AP, Stuppner H, Koeberle A (2023) Ferroptosis-modulating small molecules for targeting drug-resistant cancer: Challenges and opportunities in manipulating redox signaling
So, as you move forward, think critically about your objectives
Then the pellet was solubilized in buffer containing 20 mM HEPES, pH 7.5, 750 mM NaCl, 1% (w/v) n-dodecyl-b-D-maltoside (DDM, Anatrace), 0.2% (w/v) sodium cholate (Sigma), 0.1% (w/v) cholesterol hemisuccinate (CHS, Anatrace), 20% (v/v) glycerol, 0.2 g/ml leupeptin, 100 g/ml benzamidine, 500 unit Salt Active Nuclease (Arcticzymes) and 1 M PF-05190457 at 4 C for 3 h
Proper use of supplements that ensure proper hormonal management prevents the above problems
It's more aggressive
doi: 10.1159/000073087 155 NiwaYSumiHKawahiraKTerashimaTNakamuraTAkamatsuH